hannacore

Bacillus subtilis is a model Gram-positive bacterium, which has been widely used as industrially important chassis in synthetic biology and metabolic engineering. Fast-growing B. subtilis were constructed through rational gene knockout and adaptive laboratory evolution using wild type strain B. subtilis 168 (BS168) as starting strain. Rapid growth of chassis is beneficial for shortening the fermentation period and enhancing production of target product. The engineered B. subtilis 168 strains and strategies used for developing these strains would be useful for improving bioproduction efficiency and constructing industrially important bacterium with desired phenotype.